Annotate cells

Interactive · PANGEA
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This page conducts cell annotations on the uploaded gene expression files
This online-page is optimized for small number (~few thousand) of cells. This webpage uses users' resources, so performances can be limited by the user environment. For better and faster performance, use pangeapy API.
  1. Input file configuration
    • Should contain gene expression matrix (cell_barcode × gene_id)
    • Raw expression must be 1e4-normalized & log1p-transformed
      normalized up to 10,000 counts per cell, then log-transformed with 1 pseudocount
    • Supported formats: .csv, .csv.gz (recommended for fastest upload), or .h5ad (AnnData / HDF5)
  2. Cell annotation
    • Performed per cell barcode with a selectable model
    • PANGEA provides one Level1 model and ten Level2 models
    • Level1: 32 cell types; Level2 (combined): 165 annotations
    • Predictions are based on pre-trained logistic regression models
    • For precise results, please use the original pangeapy API
  3. Output file configuration
    • Output file: pred.csv with three columns for each cell barcode
    • predicted_label – predicted cell label from the selected model
    • conf_score – confidence score from the model prediction
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